Fig 1: RNF11 decreased in HCC tissues. (a) The expression of RNF11 in HCC tissue and paired adjacent tissue. (b) miR-425-5p expression was negatively correlated with RNF11 expression in HCC. *P < 0.05.
Fig 2: miR-425-5p directly targeted and regulated RNF11. (a) Venn diagram displaying miR-425-5p computationally predicted to target RNF11 by TargetScan, Oncomir, and miRWalk. (b) A schematic diagram of miR-425-5p putative binding sites and the corresponding mutant sites of RNF11. (c) RT-qPCR was used to examine the mRNA levels of RNF11 in HCC cells that were cotransfected with pre-miR-425-5p and miR-425-5p inhibitor. (d) The luciferase activity was used to detect the direct effect of miR-425-5p and RNF11. (e) Western blotting analysis was used to examine the protein expression of RNF11 in HCC cells that were cotransfected with pre- miR-425-5p and miR-425-5p inhibitor. *P < 0.05, **P < 0.01.
Fig 3: Restoring the expression of RNF11 rescued the miR-425-5p-mediated effect on HCC cells. (a) Western blot showed the expression of RNF11 in HepG2-miR-425-5p and Huh-7-miR-425-5p after overexpression of RNF11. (b) Effect of RNF11 on HepG2-miR-425-5p and Huh-7-miR-425-5p proliferation was analyzed using CCK-8 assays. (c) The wound healing assay was used to examine the effects of overexpression of RNF11 in HepG2- miR-425-5p. (d) The wound healing assay was used to examine the effects of overexpression of RNF11 in Huh-7-miR-425-5p. (e) Cell proliferation was detected with EDU in HepG2- miR-425-5p after overexpression of RNF11. (f) Cell proliferation was detected with EDU in Huh-7-miR-425-5p after overexpression of RNF11. *P < 0.05, **P < 0.01.
Supplier Page from Abcam for Anti-RNF11 antibody [EPR6820]